You can use ObjectFactory class - represents a static factory class for creating application objects and we have an internal method RegisterIoCTypes which is called inside Container property of type IUnityContainer which defines the behavior of the dependency injection container. So, we use IUnityContainer and Interface Injection.
Inside Application_Start event of Global.asax you can registers types with IoC framework.
You can take a look at
Inject Some Life into Your Applications—Getting to Know the Unity
Documentation on codeplex
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