Can you provide more details? Specifically
1. Clarify if the images were uploaded to a Sitefinity image library or did you use windows explorer to copy and paste the image file into a normal windows folder?
2. What do you mean by reference image? State more specifically what you want to do with the image. I'm guessing you just want to display the image on a web page.
3. Provide a sample that shows the syntax you are using to reference the image.
If you answer these questions I will probably be able to figure out the issue.